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biotinylated mal ii  (Vector Laboratories)


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    Vector Laboratories biotinylated mal ii
    Biotinylated Mal Ii, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 410 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/maackia+amurensis+lectin+ii+mal+ii/Biotinylated+Maackia+Amurensis+Lectin+II+(MAL+II)/bio_rxiv__64898__2026__04__15__718752-82-4-7
    Average 96 stars, based on 410 article reviews
    biotinylated mal ii - by Bioz Stars, 2026-10
    96/100 stars

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    Incubation:

    Article Title: O-glycan determinants regulate VWF trafficking to Weibel-Palade bodies.
    Article Snippet: .. Biotinylated lectins Peanut agglutinin (PNA) (1μg/ml) or Maackia amurensis lectin II (MAL-II) (2.5μg/ml) (both Vector Laboratories, UK) were diluted in PBS-T and incubated for 1 hour at 37°C. .. Lectin binding was detected with high sensitivity streptavidin–horseradish peroxidase (Pierce, Thermo Fisher Scientific, D ow nloaded from http://ashpublications.org/bloodadvances/article-pdf/doi/10.1182/bloodadvances.2023012499/2222704/bloodadvances.2023012499.pdf by guest on 22 April 2024 Karampini et al O-glycans influence VWF trafficking - 8 - UK) and subsequent incubation with substrate 3,3’,5,5’-Tetramethylbenzidine (TMB; R&D Systems, UK).

    Article Title: Neuraminidase is a host-directed approach to regulate neutrophil responses in sepsis and COVID-19.
    Article Snippet: Rodrigo de Oliveira Formiga | Flávia C. Amaral | Camila F. Souza | Daniel A. G. B. Mendes | Carlos W. S. Wanderley | Cristina B. Lorenzini | Adara A. Santos | Juliana Antônia | Lucas F. Faria | Caio C. Natale | Nicholas M. Paula | Priscila C. S. Silva | Fernanda R. Fonseca | Luan Aires | Nicoli Heck | Márick R. Starick | Celso M. Queiroz-Junior | Felipe R. S. Santos | Filipe R. O. de Souza | Vivian V. Costa | Shana P. C. Barroso | Alexandre Morrot | Johan Van Weyenbergh | Regina Sordi | Frederico Alisson-Silva | Fernando Q. Cunha | Edroaldo L. Rocha | Sylvie Chollet-Martin | Maria Margarita Hurtado-Nedelec | Clémence Martin | Pierre-Régis Burgel | Daniel S. Mansur | Rosemeri Maurici | Matthew S. Macauley | André Báfica | Véronique Witko-Sarsat | Fernando Spiller

    Article Title: Neuraminidase is a host‐directed approach to regulate neutrophil responses in sepsis and COVID‐19
    Article Snippet: .. Cells were incubated (37°C, 5% CO 2 ) in the presence or absence of LPS (1 μg·ml −1 , Escherichia coli 0127:b8, Sigma‐Aldrich, St. Louis, MO, USA), recombinant human (rh) S100A8 protein (2 μM, R&D Systems, Minneapolis, MN, USA), oseltamivir (100 μM, Sigma‐Aldrich) or zanamivir (30 μM, Sigma‐Aldrich) for 90 min, preincubated or not with Maackia amurensis lectin II (MAL‐II) (1 μg·ml −1 , Vector Labs, San Diego, CA, USA) or the metalloproteinase 9 inhibitor (MMP‐9i) (CAS 1177749‐58‐4, 5 nM, Calbiochem) for 30 min. ..

    Article Title: Pancreatic Cancer Cell Glycosylation Regulates Cell Adhesion and Invasion through the Modulation of α2β1 Integrin and E-Cadherin Function
    Article Snippet: .. After blocking with PBS-5% BSA containing 0.05% Tween 20 (PBST) (for sialic acids blot) or 5% low-fat milk in PBS containing 0.01% Tween 20 (for E-cadherin blot), membranes were incubated with mAb against SLe x (clone KM93, Calbiochem; 1/100) or E-cadherin (clone 36, BD Biosciences; 1/3000) in PBST-5% low-fat milk overnight at 4°C; or with biotynilated Sambucus nigra agglutinin (SNA) or biotynilated Maackia amurensis lectin II (MAL-II) (Vector Laboratories) diluted 1/200 in PBST-1% BSA for 1 h at room temperature. .. Membranes were washed and incubated with the secondary antibody HRP (horseradish peroxidase)-conjugated rabbit anti-mouse IgM (Santa Cruz Biotechnology; CA, USA) to detect SLe x , HRP-conjugated goat anti-mouse (Santa Cruz) to detect E-cadherin or Vectastain Elite ABC kit (Vector Laboratories) to detect sialic acids.

    Whole Genome Amplification:

    Article Title: Sialic acid serves as a functional receptor for grass carp reovirus.
    Article Snippet: Rabbit polyclonal antibodies against RGV major capsid protein (MCP) and envelope protein 2L was kindly provided by Professor Fei Ke (Institute of Hydrobiology, CAS). .. Alexa Fluor 594 conjugated goat anti-rabbit IgG (Cell Signaling Technology, USA), HRP-conjugated goat anti-rabbit IgG (Biosharp, China), wheat germ agglutinin lectin (WGA) (Aladdin, China), Sambucus nigra lectin (SNA) (Vector, USA), Maackia Amurensis lectin II (MAL-II) (Vector, USA), FITC-conjugated WGA (Genetex, USA), FITC-conjugated SNA (GlycoMatrix, USA), FITC-conjugated MAL-II (GlycoMatrix, USA), neuraminidase from Arthrobacter ureafaciens (Nacalai tesque, Japen), neuraminidase from Clostridium perfringens (Aladdin, China), and DAPI (Beyotime, China) were purchased from the indicated companies. ..

    Article Title: Sialic acid serves as a functional receptor for grass carp reovirus
    Article Snippet: Rabbit polyclonal antibodies against RGV major capsid protein (MCP) and envelope protein 2L was kindly provided by Professor Fei Ke (Institute of Hydrobiology, CAS). .. Alexa Fluor 594 conjugated goat anti-rabbit IgG (Cell Signaling Technology, USA), HRP-conjugated goat anti-rabbit IgG (Biosharp, China), wheat germ agglutinin lectin (WGA) (Aladdin, China), Sambucus nigra lectin (SNA) (Vector, USA), Maackia Amurensis lectin II (MAL-II) (Vector, USA), FITC-conjugated WGA (Genetex, USA), FITC-conjugated SNA (GlycoMatrix, USA), FITC-conjugated MAL-II (GlycoMatrix, USA), neuraminidase from Arthrobacter ureafaciens (Nacalai tesque, Japen), neuraminidase from Clostridium perfringens (Aladdin, China), and DAPI (Beyotime, China) were purchased from the indicated companies. ..

    Recombinant:

    Article Title: Neuraminidase is a host-directed approach to regulate neutrophil responses in sepsis and COVID-19.
    Article Snippet: Rodrigo de Oliveira Formiga | Flávia C. Amaral | Camila F. Souza | Daniel A. G. B. Mendes | Carlos W. S. Wanderley | Cristina B. Lorenzini | Adara A. Santos | Juliana Antônia | Lucas F. Faria | Caio C. Natale | Nicholas M. Paula | Priscila C. S. Silva | Fernanda R. Fonseca | Luan Aires | Nicoli Heck | Márick R. Starick | Celso M. Queiroz-Junior | Felipe R. S. Santos | Filipe R. O. de Souza | Vivian V. Costa | Shana P. C. Barroso | Alexandre Morrot | Johan Van Weyenbergh | Regina Sordi | Frederico Alisson-Silva | Fernando Q. Cunha | Edroaldo L. Rocha | Sylvie Chollet-Martin | Maria Margarita Hurtado-Nedelec | Clémence Martin | Pierre-Régis Burgel | Daniel S. Mansur | Rosemeri Maurici | Matthew S. Macauley | André Báfica | Véronique Witko-Sarsat | Fernando Spiller

    Article Title: Neuraminidase is a host‐directed approach to regulate neutrophil responses in sepsis and COVID‐19
    Article Snippet: .. Cells were incubated (37°C, 5% CO 2 ) in the presence or absence of LPS (1 μg·ml −1 , Escherichia coli 0127:b8, Sigma‐Aldrich, St. Louis, MO, USA), recombinant human (rh) S100A8 protein (2 μM, R&D Systems, Minneapolis, MN, USA), oseltamivir (100 μM, Sigma‐Aldrich) or zanamivir (30 μM, Sigma‐Aldrich) for 90 min, preincubated or not with Maackia amurensis lectin II (MAL‐II) (1 μg·ml −1 , Vector Labs, San Diego, CA, USA) or the metalloproteinase 9 inhibitor (MMP‐9i) (CAS 1177749‐58‐4, 5 nM, Calbiochem) for 30 min. ..

    Blocking Assay:

    Article Title: Pancreatic Cancer Cell Glycosylation Regulates Cell Adhesion and Invasion through the Modulation of α2β1 Integrin and E-Cadherin Function
    Article Snippet: .. After blocking with PBS-5% BSA containing 0.05% Tween 20 (PBST) (for sialic acids blot) or 5% low-fat milk in PBS containing 0.01% Tween 20 (for E-cadherin blot), membranes were incubated with mAb against SLe x (clone KM93, Calbiochem; 1/100) or E-cadherin (clone 36, BD Biosciences; 1/3000) in PBST-5% low-fat milk overnight at 4°C; or with biotynilated Sambucus nigra agglutinin (SNA) or biotynilated Maackia amurensis lectin II (MAL-II) (Vector Laboratories) diluted 1/200 in PBST-1% BSA for 1 h at room temperature. .. Membranes were washed and incubated with the secondary antibody HRP (horseradish peroxidase)-conjugated rabbit anti-mouse IgM (Santa Cruz Biotechnology; CA, USA) to detect SLe x , HRP-conjugated goat anti-mouse (Santa Cruz) to detect E-cadherin or Vectastain Elite ABC kit (Vector Laboratories) to detect sialic acids.



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    α2,3-sialylation is required for BCG-induced osteoclast differentiation and activity (A) Comparison of differentially expressed genes (DEGs) between BCG-infected osteoclasts (RB) and uninfected controls (R). (n = 3 biological replicates per group, differential expression was defined as |FoldChange| > 2 and padj <0.05). (B) Volcano plot of DEGs highlighting genes related to sialic acid biosynthesis. (C) KEGG pathway enrichment analysis of upregulated DEGs in RB cells. (D,E) Immunofluorescence staining of α2,3-SA in mouse calvarial sections <t>using</t> <t>MAL</t> <t>II</t> lectin, with quantification of α2,3-SA fluorescence intensity (n = 5). Intensity density was normalized to the PBS group mean. (F) In vitro osteoclasts subjected to MAL II lectin staining and TRAP staining, with or without sialidase treatment to enzymatically remove α2,3-SA. (G) Quantification of α2,3-SA fluorescence intensity in cultured osteoclasts (n = 5). Intensity density was measured in cellular ROIs after background subtraction and normalized to the RANKL group mean. (H) Quantification of TRAP + multinucleated cells (≥3 nuclei) per field (randomly selected fields, fixed magnification) in vitro (n = 5). (I) mRNA expression levels of osteoclast differentiation markers ( Fos, Mmp9, Nfatc1, and Ocstamp ) in osteoclasts (n = 3). Data are presented as mean ± SD. Statistical significance was determined by two-tailed unpaired Student’s t-test for two-group comparisons (E) and one-way ANOVA followed by Tukey’s post hoc test for three-group comparisons (G–I) .
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    Image Search Results


    α2,3-sialylation is required for BCG-induced osteoclast differentiation and activity (A) Comparison of differentially expressed genes (DEGs) between BCG-infected osteoclasts (RB) and uninfected controls (R). (n = 3 biological replicates per group, differential expression was defined as |FoldChange| > 2 and padj <0.05). (B) Volcano plot of DEGs highlighting genes related to sialic acid biosynthesis. (C) KEGG pathway enrichment analysis of upregulated DEGs in RB cells. (D,E) Immunofluorescence staining of α2,3-SA in mouse calvarial sections using MAL II lectin, with quantification of α2,3-SA fluorescence intensity (n = 5). Intensity density was normalized to the PBS group mean. (F) In vitro osteoclasts subjected to MAL II lectin staining and TRAP staining, with or without sialidase treatment to enzymatically remove α2,3-SA. (G) Quantification of α2,3-SA fluorescence intensity in cultured osteoclasts (n = 5). Intensity density was measured in cellular ROIs after background subtraction and normalized to the RANKL group mean. (H) Quantification of TRAP + multinucleated cells (≥3 nuclei) per field (randomly selected fields, fixed magnification) in vitro (n = 5). (I) mRNA expression levels of osteoclast differentiation markers ( Fos, Mmp9, Nfatc1, and Ocstamp ) in osteoclasts (n = 3). Data are presented as mean ± SD. Statistical significance was determined by two-tailed unpaired Student’s t-test for two-group comparisons (E) and one-way ANOVA followed by Tukey’s post hoc test for three-group comparisons (G–I) .

    Journal: Frontiers in Pharmacology

    Article Title: Mycobacterium tuberculosis infection drives osteoclast overactivation via α2,3-Sialylation to promote pathological bone destruction

    doi: 10.3389/fphar.2026.1738896

    Figure Lengend Snippet: α2,3-sialylation is required for BCG-induced osteoclast differentiation and activity (A) Comparison of differentially expressed genes (DEGs) between BCG-infected osteoclasts (RB) and uninfected controls (R). (n = 3 biological replicates per group, differential expression was defined as |FoldChange| > 2 and padj <0.05). (B) Volcano plot of DEGs highlighting genes related to sialic acid biosynthesis. (C) KEGG pathway enrichment analysis of upregulated DEGs in RB cells. (D,E) Immunofluorescence staining of α2,3-SA in mouse calvarial sections using MAL II lectin, with quantification of α2,3-SA fluorescence intensity (n = 5). Intensity density was normalized to the PBS group mean. (F) In vitro osteoclasts subjected to MAL II lectin staining and TRAP staining, with or without sialidase treatment to enzymatically remove α2,3-SA. (G) Quantification of α2,3-SA fluorescence intensity in cultured osteoclasts (n = 5). Intensity density was measured in cellular ROIs after background subtraction and normalized to the RANKL group mean. (H) Quantification of TRAP + multinucleated cells (≥3 nuclei) per field (randomly selected fields, fixed magnification) in vitro (n = 5). (I) mRNA expression levels of osteoclast differentiation markers ( Fos, Mmp9, Nfatc1, and Ocstamp ) in osteoclasts (n = 3). Data are presented as mean ± SD. Statistical significance was determined by two-tailed unpaired Student’s t-test for two-group comparisons (E) and one-way ANOVA followed by Tukey’s post hoc test for three-group comparisons (G–I) .

    Article Snippet: Biotin MAL-II , Vector laboratories , Cat# B-1265-1.

    Techniques: Activity Assay, Comparison, Infection, Quantitative Proteomics, Immunofluorescence, Staining, Fluorescence, In Vitro, Cell Culture, Expressing, Two Tailed Test